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The following results were obtained with Ni Sepharose™ 6 Fast Flow: Easy to scale-up purification from 1-ml via 5-ml to 20-ml columns. Low leakage of Ni2+ and compatibility with the reducing agent ...
Automated multistep purifi cations of five affinity tagged proteins were performed using ÄKTAxpress™, a new chromatography system. The following purification results are presented: • One ...
HisTrap™ FF crude is a ready to use column intended for purification of histidine-tagged proteins from unclarified cell lysates. No centrifugation or filtration of the samples is required before ...
Protein Structure Initiative (PSI) is a federal, university, and industry effort aimed at dramatically reducing the cost and time it takes to determine a threedimensional protein structure. Midwest ...
Affinity tags are commonly used to facilitate purification of recombinant proteins. For several applications, the affinity tag needs to be removed from the target protein. ÄKTAxpress™ is a parallel ...
Recombinant proteins can be designed to contain N- or C-terminal affinity tags. If including two different tags in a construct, the target protein can be purified utilizing different affinity media, ...
HiTrap™ IMAC HP (High Performance) is a prepacked column intended for purification of histidine-tagged or untagged proteins. It is supplied free of metal ions and is charged by the user with the ...
The new prepacked 96-well filter plates, His MultiTrap™ FF and His MultiTrap HP, offer convenient screening and rapid high-throughput purification screening of histidine-tagged proteins. His ...
High binding capacity of Ni Sepharose™ 6 Fast Flow reduces overall costs of histidine-tagged protein purification. Repeated exposure of the medium to reducing agents such as DTT did not affect ...