If isoelectric point (pI) of the target protein is known:
- select an anion exchanger (Q, DEAE, ANX) with a buffer pH above the pI.
- select a cation exchanger (S, SP, CM) with a buffer pH below the pI.
If pI is unknown:
- test for selectivity using a strong ion exchanger, Q, S or SP. Strong ion exchangers maintain their charge over a wider pH range than weak ion exchangers and are suitable for most applications.
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