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About the purification of biomolecules
Purpose of purification
Developing purification protocols
How to combine purification steps
Purification development - summary
LC techniques
Affinity Chromatography
Desalting & Gel Filtration
Hydrophobic interaction chromatography
Ion exchange chromatography
Reversed phase chromatography
Protein Purifier software
Tutorial
BioProcess™ Glossary

Purpose of purification

The purification is in itself seldom the final goal, but rather a necessary phase on the way to the final research goal to gain information about the biochemical mechanisms and structures of the biomolecules involved (Fig 1.1).

Fig 1.1. Purpose of purification.

The purification simply aims at obtaining the target molecule pure enough in the shortest possible time.

A structured way of developing a purification protocol is therefore the quickest and most reliable way to acceptable results.

When available well-designed standard purification protocols that render the target molecule suffiently pure will thus be the alternative of choice.